inhibition of il-13 by antisense oligonucleotide changes immunoglobulin isotype profile in cultured b-lymphocytes
نویسندگان
چکیده
the link between il-13 and bronchial hyper-responsiveness has brought this cytokine as a potential therapeutic target for asthma and allergic diseases. at the present study, we address the role of b cell derived il-13 in the ige and other immunoglobulin development. antisense oligo for human il-13 m-rna was used to study ige down regulation. human b-lymphocytes were purified by positive selection using magnetic cell sorting and were cultured in the complete medium plus anti-cd40 monoclonal antibody and recombinant human il-4. immunoglobulin assay was performed by elisa in the presence and absence of antisense oligonucleotide. we demonstrated that il-13 antisense causes the decrease of ige and increase of iga significantly and no significant changes in igm and igg levels (p<0.01). we also demonstrated that both il-13 inhibition and il-4 removal cause the complete blocking of ige and significant decrease of igm and igg levels. our il-13 antisense oligo can block b-cell il-13 productions and consequently inhibits ige production followed by iga class switching in vitro. we suggest that in contrast to the il-4, il-13 is apparently more potent in the ige switching and has no significant role in igg and igm levels
منابع مشابه
Inhibition of IL-13 by Antisense Oligonucleotide Changes Immunoglobulin Isotype Profile in Cultured B-Lymphocytes
The link between IL-13 and bronchial hyper-responsiveness has brought this cytokine as a potential therapeutic target for asthma and allergic diseases. At the present study, we address the role of B cell derived IL-13 in the IgE and other immunoglobulin development. Antisense oligo for human IL-13 m-RNA was used to study IgE down regulation. Human B-lymphocytes were purified by positive selecti...
متن کاملInhibition of IL-13 by Antisense Oligonucleotide Changes Immunoglobulin Isotype Profile in Cultured B-Lymphocytes
The link between IL-13 and bronchial hyper-responsiveness has brought this cytokine as a potential therapeutic target for asthma and allergic diseases. At the present study, we address the role of B cell derived IL-13 in the IgE and other immunoglobulin development. Antisense oligo for human IL-13 mRNA was used to study IgE down regulation. Human B-lymphocytes were purified by positive selectio...
متن کاملil-13 gene translation is arrested by a novel oligonucleotide in cultured human b- lymphocytes
antisense oligodeoxynucleotides (oligos) are the tools that bind to complemen¬tary sequence of targeted mrna and block specifically protein translation. in the present study, a novel 20 mer oligo as an antisense for human il-13 is introduced. this oligo is designed according to the il-13 mrna coding region and synthesized in two hplc purified and fitc conjugated forms. fluorescence oligo cell u...
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متن کاملInterleukin-10 induces immunoglobulin G isotype switch recombination in human CD40-activated naive B lymphocytes
Upon activation, B lymphocytes can change the isotype of the antibody they express by immunoglobulin (Ig) isotype switch recombination. In previous studies on the regulation of human IgG expression, we demonstrated that interleukin 10 (IL-10) could stimulate IgG1 and IgG3 secretion by human CD40-activated naive (sIgD+) tonsillar B cells. To assess whether IL-10 actually promotes the DNA recombi...
متن کاملSynthesis of IL-13 by human B lymphocytes: regulation and role in IgE production.
BACKGROUND Our laboratory has demonstrated previously that human tonsillar B lymphocytes express IL-13 mRNA OBJECTIVE: We sought to investigate IL-13 production by human B cells and the association between B cell-derived IL-13 and IgE secretion. METHODS Human B lymphocytes were isolated from tonsils and purified by means of rosetting with sheep RBCs or positive or negative selection with magn...
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عنوان ژورنال:
iranian biomedical journalجلد ۸، شماره ۴، صفحات ۱۸۵-۱۹۱
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